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lambda dna pfge molecular size standard  (Thermo Fisher)


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    Structured Review

    Thermo Fisher lambda dna pfge molecular size standard
    Lambda Dna Pfge Molecular Size Standard, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lambda dna pfge molecular size standard/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    lambda dna pfge molecular size standard - by Bioz Stars, 2026-06
    99/100 stars

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    Antibiotic resistance profiles of the five major Hong Kong MRSA  PFGE  types at the PWH

    Journal:

    Article Title: Characterization of Isolates of Methicillin-Resistant Staphylococcus aureus from Hong Kong by Phage Typing, Pulsed-Field Gel Electrophoresis, and Fluorescent Amplified-Fragment Length Polymorphism Analysis

    doi: 10.1128/JCM.41.11.4980-4985.2003

    Figure Lengend Snippet: Antibiotic resistance profiles of the five major Hong Kong MRSA PFGE types at the PWH

    Article Snippet: A lambda DNA PFGE molecular size standard (Amersham Pharmacia Biotech, Little Chalfont, United Kingdom) and an ATCC control strain were included in each gel.

    Techniques:

    PFGE profiles of SmaI macrorestriction fragments of MRSA strains. (A) eMRSA isolates. Lanes 1, 10, and 20, λ DNA PFGE markers; lane 2, ATCC 25923; lanes 3 to 9, eMRSA-1 to -7; lanes 11 to 19, eMRSA-8 to -16. (B) Representatives of international MRSA clones and major PFGE types from the PWH. Lanes 1 and 13, λ DNA PFGE markers; lane 2, ATCC 25923; lane 3, Brazilian clone (HSJ216); lane 4, Iberian clone (HPV107): lane 5, New York/Tokyo clone (BK2464); lane 6, pediatric clone (HDE288); lanes 7 to 11, representatives of major PFGE types of MRSA from the PWH (A1, B1, C1, D, and E, respectively); lane 12, internal control strain (MRSA 3).

    Journal:

    Article Title: Characterization of Isolates of Methicillin-Resistant Staphylococcus aureus from Hong Kong by Phage Typing, Pulsed-Field Gel Electrophoresis, and Fluorescent Amplified-Fragment Length Polymorphism Analysis

    doi: 10.1128/JCM.41.11.4980-4985.2003

    Figure Lengend Snippet: PFGE profiles of SmaI macrorestriction fragments of MRSA strains. (A) eMRSA isolates. Lanes 1, 10, and 20, λ DNA PFGE markers; lane 2, ATCC 25923; lanes 3 to 9, eMRSA-1 to -7; lanes 11 to 19, eMRSA-8 to -16. (B) Representatives of international MRSA clones and major PFGE types from the PWH. Lanes 1 and 13, λ DNA PFGE markers; lane 2, ATCC 25923; lane 3, Brazilian clone (HSJ216); lane 4, Iberian clone (HPV107): lane 5, New York/Tokyo clone (BK2464); lane 6, pediatric clone (HDE288); lanes 7 to 11, representatives of major PFGE types of MRSA from the PWH (A1, B1, C1, D, and E, respectively); lane 12, internal control strain (MRSA 3).

    Article Snippet: A lambda DNA PFGE molecular size standard (Amersham Pharmacia Biotech, Little Chalfont, United Kingdom) and an ATCC control strain were included in each gel.

    Techniques: Clone Assay, Control

    Correlation of phage types, FAFLP types, and  PFGE  types

    Journal:

    Article Title: Characterization of Isolates of Methicillin-Resistant Staphylococcus aureus from Hong Kong by Phage Typing, Pulsed-Field Gel Electrophoresis, and Fluorescent Amplified-Fragment Length Polymorphism Analysis

    doi: 10.1128/JCM.41.11.4980-4985.2003

    Figure Lengend Snippet: Correlation of phage types, FAFLP types, and PFGE types

    Article Snippet: A lambda DNA PFGE molecular size standard (Amersham Pharmacia Biotech, Little Chalfont, United Kingdom) and an ATCC control strain were included in each gel.

    Techniques:

    Cluster analysis of the FAFLP profiles of eMRSA-1 to -16 and representatives of the international clones performed with the Dice similarity coefficient using optimization with a position band tolerance of 0.1%. The dendrogram was calculated by UPGMA. Cluster 1 (CL1) included eMRSA-2, -5, -6, -8, and -10; CL2 consisted of eMRSA-15 only; CL3 included eMRSA-12, -13, and -14; CL4 included eMRSA-1, -4, -7, -9, and -11 and the PWH isolates of PFGE types A through E; CL5 included eMRSA-3; and CL6 included eMRSA 16. Representatives of international clones also clustered separately from eMRSA isolates.

    Journal:

    Article Title: Characterization of Isolates of Methicillin-Resistant Staphylococcus aureus from Hong Kong by Phage Typing, Pulsed-Field Gel Electrophoresis, and Fluorescent Amplified-Fragment Length Polymorphism Analysis

    doi: 10.1128/JCM.41.11.4980-4985.2003

    Figure Lengend Snippet: Cluster analysis of the FAFLP profiles of eMRSA-1 to -16 and representatives of the international clones performed with the Dice similarity coefficient using optimization with a position band tolerance of 0.1%. The dendrogram was calculated by UPGMA. Cluster 1 (CL1) included eMRSA-2, -5, -6, -8, and -10; CL2 consisted of eMRSA-15 only; CL3 included eMRSA-12, -13, and -14; CL4 included eMRSA-1, -4, -7, -9, and -11 and the PWH isolates of PFGE types A through E; CL5 included eMRSA-3; and CL6 included eMRSA 16. Representatives of international clones also clustered separately from eMRSA isolates.

    Article Snippet: A lambda DNA PFGE molecular size standard (Amersham Pharmacia Biotech, Little Chalfont, United Kingdom) and an ATCC control strain were included in each gel.

    Techniques: Clone Assay